Ability of foot-and-mouth disease virus to form plaques in cell culture is associated with suppression of alpha/beta interferon.

نویسندگان

  • J Chinsangaram
  • M E Piccone
  • M J Grubman
چکیده

A genetic variant of foot-and-mouth disease virus lacking the leader proteinase coding region (A12-LLV2) is attenuated in both cattle and swine and, in contrast to wild-type virus (A12-IC), does not spread from the initial site of infection after aerosol exposure of bovines. We have identified secondary cells from susceptible animals, i.e., bovine, ovine, and porcine animals, in which infection with A12-LLV2, in contrast to A12-IC infection, does not produce plaques; this result indicates that this virus cannot spread from the site of initial infection to neighboring cells. Nevertheless, A12-LLV2 can infect these cells, but cytopathic effects and virus yields are significantly reduced compared to those seen with A12-IC infection. Reverse transcription-PCR analysis demonstrates that both A12-LLV2 and A12-IC induce the production of alpha/beta interferon (IFN-alpha/beta) mRNA in host cells. However, only supernatants from A12-LLV2-infected cells have significant antiviral activity. The antiviral activity in supernatants from A12-LLV2-infected embryonic bovine kidney cells is IFN-alpha/beta specific, as assayed with mouse embryonic fibroblast cells with or without IFN-alpha/beta receptors. The results obtained with cell cultures demonstrate that the ability of A12-IC to form plaques is associated with the suppression of IFN-alpha/beta expression and suggest a role for this host factor in the inability of A12-LLV2 to spread and cause disease in susceptible animals.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Assessment of Foot and Mouth Virus Subtype O2016 Genetic Alterations During Successive Passages in BHK Monolayer

Abstract : Foot and Mouth Disease is one of  the important live stocks contagious viral disease caused by Aphtovirus genus ,  that is belong to family RNA virus  picornaviride. The important characteristic of FMD virus is high mutation that give rise to diversity of Antigen in surface of Neutralizing proteins. For this reason FMD virus have 7 distinct serotype and many subtype. Vaccination is o...

متن کامل

An uncommon clinical form of foot-and-mouth disease in beef cattle presented with cornual skin lesions

Foot-and-mouth disease (FMD) is a major infectious disease in livestock. The common clinical signs in cattle include epidermal vesicles that are majorly distributed around oronasal cavity, feet and teats. The aim of this report is to document an uncommon clinical form of the disease which comprises the occurrence of classic vesicular lesion in a rarely observed location of the horn vegetative t...

متن کامل

طراحی، ساخت و بیان ‌اپی‌توپ‌های پروتئین VP1 ویروس تب برفکی سروتیپO ایران

     Foot-and-mouth disease (FMD) is an extremely infectious and occasionally fatal viral disease with a rapid onset and a short course that affects cloven-hoofed animals and results in considerable financial losses. For this reason, availability of more reliable and efficient vaccines against Foot-and-mouth disease virus (FMDV) is felt as a necessity. The purpose of this study is to develop a ...

متن کامل

Assessment of the Immunogenicity of Foot and Mouth Disease Vaccine Produced by Razi Institute against Types of A13, A15 and O2010 of FMD Virus

Background and Aims: Foot-and-Mouth Disease (FMD) is a highly contagious infectious disease of livestock which has made a barrier to hygiene causing severe loss in livestock and their products. The aim of this study was the assessment of antibody response against foot and mouth disease virus  types A13, A15 , O2010, after injection of FMD vaccine candidate produced by Razi Institue. Mater...

متن کامل

Developing Vaccines against Foot-and-Mouth Disease: a Biotechnological Approach

Foot-and-mouth disease (FMD) is a contagious viral disease of livestock with significant economic effect. It is prevalent in various regions of Asia, Africa, and South America. The causative agent of this disease is called foot-and-mouth disease virus (FMDV), which is a member of Aphthovirus genus. Vaccination is an effective technique to prevent the complications of FMD and to eradicate the di...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of virology

دوره 73 12  شماره 

صفحات  -

تاریخ انتشار 1999